3.3 Salk phenotypes

M 3/5/12

Per Pair:

• Control seeds (aliquot ~25 into 12 microfuge tubes, dry)

• Salk-line seeds (remainder from Lab 3.2, dry, ~25 per tube)

• 2 mL 70% EtOH, 0.05% TritonX-100 (stock is 10%)

  • Make 50 mL, aliquot into microfuge tubes

  • 37 mL 95% EtOH

  • 0.25 mL 10% Triton-X
  • water to 50 mL

• If seeds are to be plated in agar:

  • 0.1% agar, sterile
  • ~ 2 mL per pair
  • 0.1 g agar/100 mL, autoclave, aliquot.
  • Or aliquot into glass tubes, then autoclave

• sterile water

• If seeds are to be dried and plated from filter paper:

  • Filter paper (? 4 in a plastic Petri dish?)
  • Sterile forceps in tubes for handling filter paper
  • 10 mL 95% EtOH (aliquot 25 15mL tubes)

• 2 complete medium plates per pair

• 2 Low-Fe medium plates (1 µM Fe) per pair

• micropore tape

Total plates (actually need 20 of each type)

• 24 complete MS medium

• 24 low Fe MS medium