Skip to main content

User account menu

Show — User account menu Hide — User account menu
  • Log in
Home
ISB Server Wahoo
Providing File, Print, Radmind, and Other Services

Main navigation

Show — Main navigation Hide — Main navigation
  • Home

Breadcrumb

  1. Home
  2. Gene & Genome

PCR Calcs

Submitted by kdorfman on Mon, 04/01/2013 - 20:56

From Takara Ex Taq recipe

For PCR reactions
at µL per reaction
addµL 10X Ex Taq buffer
addµL dNTP 2.5 mM
addµL forward primer 12.5 µM
addµL reverse primer 12.5 µM
addµL template DNA (~250 ng/µL)
addµL water to final volume
addµL ExTaq 1U/µL
for a final volume ofµL

Book traversal links for PCR Calcs

  • ‹ Arabidopsis resources
  • Up
  • Readings & on-line resources ›
  • Printer-friendly version
  • Log in to post comments
pcrcalcs.xls
TaKaRa ExTaq manual

Show — Hide —
  • Accounts and Passwords
  • Print Release System
  • Better Labs and Prep Rooms
  • Bioimaging Instructor
  • QBoC
  • Research Methods
  • Markdown & Code Help
  • Gene & Genome
    • !2022 (Maresca)
    • 2022 (Arabidopsis)
    • Course Prep
    • 0.1 Micro-mixology
    • 0.3 Arabidopsis anatomy
    • 1.1 DNA extraction
    • 1.2 DNA Quantification
    • 2.1 - 2.3 Gene maps
    • 2.4 Testing PCR
    • 2.5 Restriction Mapping
    • 2017 schedule
    • 2018
    • 2021 DNA Extraction
    • 2021 RNA extraction
    • 2021 RT & qPCR
    • 3.2 Planting Salk seeds
    • 3.3 Salk phenotypes
    • 3.4 Leaf Squish & PCR
    • 3.5 Salk genotypes
    • 4. Bioinformatics
    • 5.1 - 5.3 expression
    • 5.4 RNA Extraction
    • 5.5 RT-PCR
    • 5.6 RT-PCR analysis
    • 5.6 qPCR
    • Arabidopsis resources
    • PCR Calcs
    • Readings & on-line resources
  • Genetics Lab
  • QSB Riley
  • Histology
  • Model Systems
  • 2014
  • Genetics & Evolution lab
  • NBP
  • Genetics Genomics & Evolution
  • CMBL 2022
  • S2 Cells prep
Powered by Drupal