Saburaud dextrose agar

Submitted by kdorfman on Fri, 04/14/2023 - 18:19

Wikipedia entry

Before you start:

  • check for 20% glucose, filter sterilized; make more if needed
  • warm the glucose (a ~60C oven is best; use a 37 incubator if necessary)
  • sterilize a graduated cylinder if volume of glucose to be added is more than 50 mL

per L:

  • 40 g glucose
  • 10 g peptone
  • 20 g agar
  • pH 5.6
To make mL Saburaud
start with: mL water
add: grams peptone
adjust pH to 5.6
bring volume tomL water
add: grams agar
autoclave for: minutes
aseptically add: mL sterile 20% glucose