Master mix

Submitted by kdorfman on Mon, 06/12/2023 - 15:05
To make Qiagen Master Mix + primers for students
and reactions per student
at uL per reaction
Start with: uL Qiagen Master Mix
add: uL forward eGFP primer (100 mM)
add: uL reverse eGFP primer (100mM)
add: uL forward Hedgehog Inhibitory Protein primer (100 mM)
add: uL reverse Hedgehog Inhibitory Protein primer (100 mM)
add: uL water
for a final volume of: uL Qiagen Master Mix plus primers
aliquot: uL Qiagen Master Mix plus primers per student