ER-tracker

Submitted by kdorfman on Tue, 11/22/2011 - 14:22

ER-Tracker from Invitrogen

E34250 ER-Tracker™ Red (BODIPY® TR glibenclamide) for live-cell imaging 100 μg

1mM Stock

Add 110 µL DMSO to the 100 µg in vial.

  • Aliquot 1 µL

  • Freeze

1 µM working concentration

  • add 0.999 mL HBSS/Ca/Mg

Protocol

  • rinse with warm HBSS

  • treat with 1 mL warm 1 µM ER-tracker

  • incubate 15-30 min at 37C

To view stained cells

  • rinse with medium

  • view with fluorescence microscope

To fix cells

  • 4% formaldehyde, for 2 min, at 37C

  • rinse 2x in buffer - NO TRITON X-100

er-tracker.pdf