Cell passaging

Submitted by kdorfman on Wed, 09/29/2021 - 17:07

Students split a flask of B16s into a new flask and a coverslip-bottom dish for viewing next class

  • 1 flask of B16-30 (wild type) per pair (at least 50% confluent)

  • 1 flask, 1 coverslip-bottom dish per student

  • 1 50 mL tube of DMEM per pair

  • 1 15 mL tube of PBS per pair

  • 1 0.5 mL aliquot of trypsin per pair

  • 5 mL serologicals

  • sterile transfer pipettes

  • 70% ethanol spray bottles

  • lots of gloves

  • handout