Submitted by kdorfman on Wed, 08/31/2016 - 20:24

Lilly pollen growth medium

205 mM (7%) sucrose (See Sugars.)

1.6 mM H3BO3

0.1 mM CaCl2

15 mM MES

To make mL LPGM
at % sucrose
add: mL 150 mM MES
add: uL 10 mM CaCl2
add: uL 160 mM Borate, pH to 5.7 with KOH
add: g sucrose

Either make fresh each day, or filter sterilize for all the labs.

Stock solutions:

150 mM MES

10 mM CaCl2

160 mM Borate