Taxol

Taxol (20 µM final)

Fisher 109710 Paclitaxel.
Sigma T7402 1mg
MW = 853.91

• Stabilizes the microtubule polymer
• Protects microtulues from disassembly.
• Prevents metaphase spindle configuration

• Blocks progression of mitosis and prolonged activation of the mitotic checkpoint triggers apoptosis or reversion to the G-phase of the cell cycle without cell division.

• soluble in DMSO, not water.

• Add 0.117 mL DMSO to make a 0.01M (=10 mM) stock solution from the 1 mg powder

• Aliquot 10 µL 10 mM (if it precipitates, add 10 uL DMSO and vortex, then add 480 uL medium or buffer)

  • add 490 µL to make 200µM
  • dilute into medium from there (100 uL Taxol/mL medium)