STLC

• STLC Sigma 164739 (Fisher 50-703-1833), MW = 363.47

(+)-S-Trityl-L-cysteine is a cell-permeable selective inhibitor of mitotic kinesin Eg5 and ATPase activities.

KIF11 (also known as kinesin-5 and Eg5) is a homotetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. The motor domain or motor head is at the N-terminus and performs ATP hydrolysis and binds to microtubules. (https://en.wikipedia.org/wiki/Kinesin_family_member_11)

• Make 100 mM stock:

  • 1 g in bottle.
  • Mix with 27.5 mL DMSO
  • heat to 65C (~10 min), vortex
  • if necessary, filter sterilize to remove insoluble particles.
  • save as 1 mL aliquots in freezer. (dilute 1:10,000 to use) (dilute 1:100 to make 1 mM)

• Make 1 mM stock from the 100 mM stock

  • 10 µL 1mM stock
  • 990 µL DMSO
  • vortex
  • it will crystallize in the refrigerator. Warm (60C) and vortex to redissolve it. DO NOT LEAVE IN HOT BLOCK - IT WILL TURN BLACK!

• Make 10 µL aliquots of 1 mM stock. Freeze

  • label says to add 990 µL medium to make 10 µM working solution