HU (Hydroxyurea)

Submitted by kdorfman on Wed, 01/06/2021 - 18:29
ingredient working conc MW stock
HU 0.2 M 76 Koshand lab 2M in H2O

Hydroxyurea: (Sigma H8267-1g)

  • To make 50 mL YPD with 0.2M HU

  • vi = (0.2M * 50 mL)/2M = 5 mL

  • 100µL 2M stock per mL medium

  • Buy 1 g, make 6.6 mL 2M stock

  • MW = 76, so 1 g = 1/76 mol = 0.0132 mol
  • vol = (1000 mL/2 mol) * 0.0132 mol = 6.6 mL

See yeast recipes for QBoC https://wahoo.cns.umass.edu/node/554/