Submitted by kdorfman on Thu, 08/17/2023 - 20:00 from Kathleen Arcaro To make mL D5 DMEM with pyruvate, glucose, glutaminestart with mL wateradd: g DMEM (Gibco 12800-058/ fisher 12800017)add: g NaHCO3 if there is none already in the DMEMadd: g HEPES, then pH to 7.2 (initial pH = ~7.1)add: mL insulin solution 10 mg/mL in HEPESadd: mL amino acid solutionadd: mL cosmic calf serumadd: mL anti/anti bring to final volume, filter sterilize in BSC, refrigerate insulin solution 10 mg/mL in HEPES amino acid solution Book traversal links for DC5 (DMEM for canine cancer) ‹ Antibiotic/Antimycotic Up DMEM › Printer-friendly versionLog in to post comments DMEM for Canine Cancer Cells