DC5 (DMEM for canine cancer)

Submitted by kdorfman on Thu, 08/17/2023 - 20:00

from Kathleen Arcaro

To make mL D5 DMEM with pyruvate, glucose, glutamine
start with mL water
add: g DMEM (Gibco 12800-058/ fisher 12800017)
add: g NaHCO3 if there is none already in the DMEM
add: g HEPES, then pH to 7.2 (initial pH = ~7.1)
add: mL insulin solution 10 mg/mL in HEPES
add: mL amino acid solution
add: mL cosmic calf serum
add: mL anti/anti

bring to final volume, filter sterilize in BSC, refrigerate

insulin solution 10 mg/mL in HEPES

amino acid solution