P-Glo Bacterial media

Make 60 mm plates

• plain LB plates

• LB/Amp (or Carb) plates (1 black stripe)

• LB/Amp/Ara plates (1 black, 1 red stripe)

2019 - for QBoC:

• transformed pglo plasmid (1660405EDU) from the BioRad kit into
• DH5 alpha E. coli (Invitrogen 18265-017) from the -80
• Lyophilized cells (BioRad 1660408EDU) did not arrive in time

Used the Invitrogen protocol.

Plated on:

• LB
• LB amp
• LB amp ara

Saw gfp colonies on the arabinose plates. Collected, grew up in LB amp, froze in 15% glycerol.