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Enzyme |
Cut Smart |
NEB 1 |
NEB 2 |
NEB 3 |
NEB 4 |
---|
Aat2 |
100 |
10 |
50 |
50 |
|
Acc651 |
25 |
10 |
75 |
100 |
|
Aci1 |
100 |
10 |
25 |
100 |
|
Acl1 |
100 |
10 |
10 |
10 |
|
AflII |
100 |
50 |
100 |
10 |
|
Age1 |
75 |
100 |
75 |
25 |
|
AlwN1 |
100 |
10 |
100 |
50 |
|
Apal1 |
100 |
100 |
100 |
10 |
|
Asc1 |
100 |
10 |
10 |
10 |
|
Ava1 |
100 |
10 |
100 |
25 |
|
Ava2 |
100 |
50 |
75 |
10 |
|
BamHI-HF |
100 |
100 |
50 |
10 |
|
BciVI |
100 |
100 |
25 |
10 |
|
Bgl2 |
10 |
10 |
10 |
100 |
|
BmgB1 |
10 |
10 |
10 |
100 |
|
BmsAI |
100 |
50 |
100 |
100 |
|
BsaI |
100 |
75 |
75 |
100 |
|
BspD1 |
100 |
25 |
75 |
50 |
|
BspE1 |
10 |
10 |
10 |
100 |
|
BsrB1 |
100 |
50 |
100 |
100 |
|
BsrG1 |
25 |
25 |
100 |
100 |
|
Cla1 |
100 |
10 |
50 |
50 |
|
Dra1 |
100 |
75 |
75 |
50 |
|
Eag1 |
10 |
10 |
25 |
100 |
|
Ear1 |
100 |
50 |
10 |
10 |
|
EcoR1 |
50 |
25 |
100 |
50 |
|
EcoRV-HF |
100 |
25 |
100 |
100 |
|
HaeIII |
100 |
50 |
100 |
25 |
|
Hha1 |
100 |
25 |
100 |
100 |
|
Hind3 |
50 |
25 |
100 |
50 |
|
Hind3 HF |
100 |
10 |
100 |
10 |
|
HinP1I |
100 |
100 |
100 |
100 |
|
Hpa1 |
100 |
10 |
75 |
25 |
|
Hpa2 |
100 |
100 |
50 |
10 |
|
Kas1 |
100 |
50 |
100 |
50 |
|
KpnI-HF |
100 |
100 |
25 |
10 |
|
Mbo1 |
100 |
75 |
100 |
100 |
|
Mlu1 |
25 |
10 |
50 |
100 |
|
Msp1 |
100 |
75 |
100 |
50 |
|
Nae1 |
100 |
25 |
25 |
10 |
|
Nco1 |
100 |
100 |
100 |
100 |
|
Nde1 |
100 |
75 |
100 |
100 |
|
Nhe1-HF |
100 |
100 |
25 |
10 |
|
Pst1 |
50 |
75 |
75 |
100 |
|
Pvu1 |
10 |
10 |
25 |
100 |
|
Pvu2 |
100 |
50 |
100 |
100 |
|
Rsa1 |
100 |
25 |
50 |
10 |
|
Sac1 |
100 |
100 |
50 |
10 |
|
Sal1 |
10 |
10 |
10 |
100 |
|
Sau3A1 |
100 |
100 |
50 |
10 |
|
Sca1 |
NR |
NR |
NR |
100 |
|
SexA1 |
100 |
100 |
75 |
50 |
|
Sma1 |
100 |
10 |
10 |
10 |
|
Spe1 |
100 |
75 |
100 |
25 |
|
Sph1 |
100 |
100 |
100 |
50 |
|
SSpI |
50 |
50 |
100 |
50 |
|
Sty1 |
10 |
10 |
25 |
100 |
|
Sty1-HF |
100 |
25 |
100 |
25 |
|
Xba1 |
100 |
10 |
100 |
75 |
|
Xcm1 |
100 |
10 |
100 |
25 |
|
Xho1 |
100 |
75 |
100 |
100 |
|