Make solutions (Check supplies first)
- Tris pH8 1M 25 mL
- Tris pH7.5 1M 25 mL
- EDTA 500mM 200 mL
- NaOAc 3M 10 mL
- KOAc 5M 100 mL
- SDS 20mM 25 mL
- NaCl 5M 25 mL
- DEB 200 mL
- T10E1 100 mL
- T10E5 10 mL
- TAE 50x 1 L
- MassRuler™ DNA Ladder, High Range Fermentas #SM0393 (not 2018?)
- MassRuler™ DNA Ladder, Low Range Fermentas #SM0383 (not 2018?)
- MassRuler™ DNA Ladder Mix, SM0403 FERSM0403 (print 2 copies to put on the gel docs)
- 6X loading dye:
- 30% glycerol
- 0.3% Bromphenol blue
- 0.3% xylene cyanol
Aliquot:
| Solution | vol | aliquots/pair | total aliquots | for Labs | |
|---|---|---|---|---|---|
| T10E1 | 1 | mL | 5 | 50 | 1.1, 1.2, 2.4, 3.3 |
| T10E5 | 0.75 | mL | 2 | 20 | 1.1 |
| EtOH 95% | 1.5 | mL | 2.2 | 22 | 1.1, 5.4 |
| EtOH 70% | 7.5 | mL | 1.2 | 12 | 1.1 |
| isopropyl | 7.5 | mL | 1.2 | 12 | 1.1 |
| NaOAc | 100 | µL | 1.2 | 12 | 1.1 |
| KOAc | 5 | mL | 1.2 | 12 | 1.1 |
| DEB | 10 | mL | 1.2 | 12 | 1.1 |
| Tris pH 7.5 10 mM | 1.5 | mL | 3 | 30 | 1.2, 5.4 |
| loading dye | 50 | µL | 1.5 | 15 | 1.2, 2.4, 2.5, 3.4, 5.4, 5.6 |
| HMW std | 15 | µL | 1.2 | 12 | 1.2 |
| dNTP 2.5 mM | 150 | µL | 1.5 | 15 | |
| sterile H2O | 1 | mL | 6 | 60 | 2.4, 2.5, 3.3, 5.5 |
| LMW std | 50 | µL | 1.5 | 15 | 2.4, 2.5, 3.4, 5.4, 5.6 |
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