On soil

Arabidopsis planting guide - Plants on soil

Routine weekly planting to produce plant material for observation or DNA extraction

• Pots
  • 6 pots in a big flat with no holes
  • fill with clump-free potting soil, leaving some space at the rim
• Treat with Gnatrol for fungus gnats
  • saturate with hot water the night before Teddi comes
  • Arrange for Teddi to do Gnatrol treatment
• OR Heat in oven
  • pre-heat oven to 225F (=~107C)
  • Put soil-filled pots in aluminum lasagne pan
  • Cover with foil
  • insert thermometer in one pot near center
  • Heat to 180F (=~82C)
  • leave in oven for 30 min.
  • leave covered until ready to plant
• Seeds for DNA
  • Start a set 4 weeks before needed, another set 3 weeks before
  • Columbia wild type (col-0)
  • Scatter seeds on surface of soil (remoisten before if it is dry)
  • Put on plastic cover
  • Put in refrigerator, keep dark (this synchronizes germination)
  • Transfer to growth chamber on the third day
  • If no room in fridge, put seeds in microfuge tube, add water, refrigerate in dark 2 days, then plant
• Seeds for demo or experimental plants
  • Put seeds in microfuge tube, water, refrigerate, keep dark
  • 3rd day: pour into small beaker, add more water
  • Deposit one seed to each corner of the pot, plus one in the middle with a transfer pipet.
  • Transfer to growth chamber
• Growth Chamber
  • 22C, 16H light, schedule continuous program
  • Water bottom flat ~3x per week
  • Remove cover after plants are well established.