Immunostaining RNAi S2 cells

Submitted by kdorfman on Fri, 09/30/2022 - 15:49

W 10/12/22

  • 1 con-A coverslip in a dish per student + backups (repeat day: 2 per group + backup)
  • coverslip forceps
  • 4% PFA
  • PBS-Tw-Azide
  • Rabbit anti-Phospho H3
  • Rat anti-tubulin
  • goat anti rabbit red (Cy3)
  • goat anti rat 488
  • DAPI mounting medium
  • nail polish