HeLa for 2nd day G&GA

9 HeLa (Vikash) coverslips

Fixed in paraglut

Stained for phospho H₃

• rabbit anti PHH3 (1:2000)
• goat anti-rabbit FITC (1:80)

Stained for alpha tubulin:

• rat anti tubulin (directions on tube)
• goat anti rat TRITC (1:1000)

Mounted in DAPI

Note: Red tubulin was gorgeous! Green PHH3 not so much