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4.2 2013

Submitted by kdorfman on Thu, 09/26/2013 - 14:43

Tu 10/15 & W 10/16

Mechanism of Motility

Students check for effect of dose and time on actin filaments. Treat LL parentals with cytochalasin D, then stain actin with phalloidin.

  • LL on coverslips

  • non-CO2 medium

  • Cytochalasin D

  • paraglut

  • PBS-Tw-Az

Phalloidin

50 µL/ coverslip (15 min at RT)

2 coverslips per group

100 µL/group

aliquoted to make 100 µL. Directions on tube.

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