LL's for student slides
Fix 90 in formaldehyde; 10 in methanol
Plate 102 in 17 6-well plates.
| Lab | # coverslips | fix | stain | secondary |
|---|---|---|---|---|
| 1.1 | 10 | form or paraglut | ||
| 1.2 | 10 | " | ||
| 1.3 | 10 | " | ||
| 2.1 | 10 | " | anti-tubulin, phalloidin | goat anti-rat |
| 2.2 | 10 | " | anti-tubulin | goat anti-rat |
| 3.1 | 10 | " | anti-tubulin, phalloidin FRESH | goat anti-rat |
| 3.2 | 3 | form | alpha actinin | anti-mouse IgM |
| 3.2 | 3 | form | anti Golgi | anti-mouse IgG |
| 3.2 | 3 | form | vinculin | |
| 3.2 | 3 | MeOH | anti gamma-tubulin | |
| 3.2 | 3 | " | anti-Hec1 | |
| 3.2 | 3 | " | LAP2 | |
| 3.3 | 20 | form or paraglut | un-mounted for students to stain |
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