Concanavalin-coated coverslip dishes
Start with 5 mg/mL ConA stock solution
Keep sterile - work in sterile hood
aliquot 100 µL into microfuge tubes (cannot take repeated freeze-thaw cycles)
Coat coverslip bottom dishes with either:
- 0.75 mL 0.1 mg/mL: 0.015 mL (5 mg/mL) + 0.735 mL PBS
- 0.75 mL 0.5 mg/mL: 0.075 mL (5 mg/mL) + 0.675 mL PBS
Incubate the coverslip dishes for 1 hour 37˚C
Remove the solution
rinse in PBS
Air dry the dish.
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