M21 from stocks

Submitted by kdorfman on Wed, 12/15/2021 - 21:19
To make plates (100 mm)
(for a final volume of mL of medium)
start with: mL water
add: mL 1M K2HPO4
add: mL 1M KH2PO4
add: g Difco Bacto Peptone, and bring to final volume
add: g agar
autoclave for: minutes

Add 50 - 100 µg/mL ampicillin (carbenicillin) and 1 mM IPTG if needed