3-color LD + SYBR Safe

Submitted by kdorfman on Mon, 10/15/2018 - 17:38

The orange runs fast; suitable for small DNA fragments

To make 10 mL from home made LD:

  • 6 mL orange G (Fisher AAJ60562AC)
  • 1 mL home made LD with bromophenol blue and xylene cyanacol
  • 6 uL SYBR safe
  • 1 mL glycerol
  • 0.1 mL 1 M Tris pH 7.6
  • water to 10 mL