Sterilize seeds with EtOH then dry

Submitted by kdorfman on Mon, 02/14/2022 - 18:39
  • Put appropriate number of seeds in a sterile microfuge tube

  • Sterilize filter paper with EtOH, let dry in sterile hood (can set it on top of an open sterile petri plate cover

  • Add 95% EtOH to seeds for 5 min, dump seeds onto filter paper

  • let dry, tap onto agar of an MS plate