- Add ~1mL of bleach solution to each tube
- Cap and shake
- Leave tube on its side for 20-30 min, shaking periodically
- Put upright after last shake (so seeds settle)
- In laminar flow hood
- Use a pipetman that has been sprayed with ethanol and air-dried in the hood
- Remove as much bleach solution as possible from the tube
- Add ~1 mL sterile ddH2O, mix and let seeds settle again
- Do at least 4 washes, to dilute out the bleach
- Add ~1mL sterile 0.1% agarose (so seeds will be suspended in the liquid)
Bleach solution for sterilizing seeds
- 7 mL sterile ddH2O (must be freshly made!)
- 3 mL Chlorox
- 1 uL Triton X-100 (detergent to help get bleach into seed crevices)
0.1% agarose for plating seeds
- 100 mL sterile ddH2O
- 0.1 g agarose . autoclave to melt; shake bottle after autoclaving
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