5.1 DNA extraction for genotyping

From Elizabeth Vierling

Per Reaction:

• 500 uL Edwards Extraction Buffer (6.5 mL/pair, 100 mL/class)

• 300 uL Isopropanol (3.9 mL/pair, 50 mL/class)

• 100 uL T10E1 (1.3 mL/pair, 20 mL/class)

• 1 2-mL grinding tube (12 tubes/pair, 50/class)

• 2 steel beads (24 beads/pair, 300 beads/class)

Per class * Dresch ball mill grinder

• 6 microcentrifuge

• 6 vortex