6.4 RNA extraction 2022

Submitted by kdorfman on Tue, 04/19/2022 - 14:18

RNA Extraction

Materials for the class:

  • 6 centrifuges
  • Tissuelyzer blocks in the -80
  • LN2
  • Freezer box to keep RNA in the -80

Materials per group:

  • scissors
  • ice bucket
  • jeweler's scale
  • 6 grinding tubes with 2 metal beads each
  • 6 lilac columns
  • 6 pink columns
  • 6 capless collection tubes

Reagents per group:

  • ~3 mL RLT + β-Mercaptoethanol
  • 1.5 mL 95% Ethanol
  • 4.2 mL buffer RW1
  • 6 10 uL aliquots DNAse
  • 420 uL buffer RDD
  • 6 mL buffer RPE
  • 300 uL RNase free water