RNA Extraction
Materials for the class:
- 6 centrifuges
- Tissuelyzer blocks in the -80
- LN2
- Freezer box to keep RNA in the -80
Materials per group:
- scissors
- ice bucket
- jeweler's scale
- 6 grinding tubes with 2 metal beads each
- 6 lilac columns
- 6 pink columns
- 6 capless collection tubes
Reagents per group:
- ~3 mL RLT + β-Mercaptoethanol
- 1.5 mL 95% Ethanol
- 4.2 mL buffer RW1
- 6 10 uL aliquots DNAse
- 420 uL buffer RDD
- 6 mL buffer RPE
- 300 uL RNase free water
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