Check extracts for DNA
- 1% gels
- HW Massruler
Amplify extracted DNA 24 PCR reactions per pair (12 WT + 12 Salk)
Reagent | WT allele master mix uL | mutant master mix uL | per pair | per class |
---|---|---|---|---|
H2O | 243.25 | 243.25 | 486.5 | ~6 mL |
10X ex taq buf | 35 | 35 | 70 | ~145 uL |
dNTP | 28 | 28 | 60 | 720 uL |
LBP | 17.5 | 17.5 | 210 uL | |
GSP 1 | 17.5 | ? | ||
GSP 2 | 17.5 | ? | ||
Taq 5U/uL | 1.75 uL | 1.75 uL | 3.5 uL | 42 uL |
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