M 3/5/12
Per Pair:
Control seeds (aliquot ~25 into 12 microfuge tubes, dry)
Salk-line seeds (remainder from Lab 3.2, dry, ~25 per tube)
2 mL 70% EtOH, 0.05% TritonX-100 (stock is 10%)
Make 50 mL, aliquot into microfuge tubes
37 mL 95% EtOH
- 0.25 mL 10% Triton-X
- water to 50 mL
If seeds are to be plated in agar:
- 0.1% agar, sterile
- ~ 2 mL per pair
- 0.1 g agar/100 mL, autoclave, aliquot.
- Or aliquot into glass tubes, then autoclave
sterile water
If seeds are to be dried and plated from filter paper:
- Filter paper (? 4 in a plastic Petri dish?)
- Sterile forceps in tubes for handling filter paper
- 10 mL 95% EtOH (aliquot 25 15mL tubes)
2 complete medium plates per pair
2 Low-Fe medium plates (1 µM Fe) per pair
micropore tape
Total plates (actually need 20 of each type)
24 complete MS medium
24 low Fe MS medium
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Watch plants throughout the semester
Change the lab manual so it says they have to follow any homozygous or heterozygous Salk plants throughout their entire life - till the end of the semester.