RT
6 RNA extractions, 6 cDNA reactions/group; 23 groups
- 65C (thermocycler)
- pcr strips
- ice
Give each group enough for 7 reactions
Reagent | uL per rxn | uL per 7 rxns |
---|---|---|
50 uM oligo(dT) | 1 | 7 |
RNA | ||
10 mM dNTP | 1 | 7 |
H2O | 11 | 71.5 |
5X 1st strand buffer | 4 | 28 |
0.1M DTT | 1 | 7 |
RNAse out | 1 | 7 |
Superscript III | 1 | 7 |
qPCR
48 q pcr reactions/group (24 GOI, 24 HK) Give them enough for 50 reactions
Reagent | uL per rxn | uL per 50 rxns |
---|---|---|
2x SYBR Green master mix | 5 uL | 250 uL |
water | 5 uL | 250 uL |
forward primer | 0.5 uL | |
reverse primer | 0.5 uL | |
cDNA | 1 uL |
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