Make enough for 2 gels/pair 1.5% agarose
gels | mL TAE | g agarose | µL EtBr |
---|---|---|---|
11 | 550 | 8.25 | 5.5 |
20 | 1000 | 15 | 10 |
22 | 1100 | 16.5 | 11 |
24 | 1200 | 18 | 12 |
Use EtBr TAE
Use LMW mass ruler
Prepare 15 μL of each of your PCR reactions for loading on gels by transferring to a clean, labeled 1.5 mL microfuge tube, and adding 3 μL of loading dye. Load the entire 18 μL – it is important that all lanes contain the same amount.
Load gels, and run at 100V for 45 minutes. Remember to load 5 μL (= 220 ng) of LMW Mass Ladder as your standard.
Analyze gels
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