Submitted by kdorfman on Mon, 04/01/2013 - 20:56 From Takara Ex Taq recipe For PCR reactions at µL per reaction addµL 10X Ex Taq buffer addµL dNTP 2.5 mM addµL forward primer 12.5 µM addµL reverse primer 12.5 µM addµL template DNA (~250 ng/µL) addµL water to final volume addµL ExTaq 1U/µL for a final volume ofµL Book traversal links for PCR Calcs ‹ Arabidopsis resources Up Readings & on-line resources › Printer-friendly versionLog in to post comments pcrcalcs.xls TaKaRa ExTaq manual