| Week | Day | Date | Lab |
|---|---|---|---|
| 1 | W | 1/26 | 0.1: Plant Col0 seeds (24 pots), micropipetting |
| 2 | M | 1/31 | 0.2: Benchling platform 1.1: Initial working maps |
| W | 2/02 | 1.2: Finalizing gene models | |
| 3 | M | 2/07 | 1.3: Identifying T-DNA insertion sites |
| W | 2/09 | 1.4: Designing primers | |
| 4 | M | 2/14 | 2.1: DNA extraction (plants from week 1) Lab 1 report due |
| W | 2/16 | 2.2: DNA quantification | |
| 5 | Tu | 2/22 | 4.1: BLAST |
| W | 2/ 23 | 4.2: Literature search | |
| 6 | M | 2/28 | 2.3: Testing PCR conditions |
| W | 3/02 | 0.3: Arabidopsis essentials (6 rose pots per table) 3.1: Salk like genetics plant Salk seeds (48 pots) Lab 2 report due |
|
| 7 | M | 3/07 | 4.3: Phylogeny |
| W | 3/09 | Catch-up Day | |
| SPRING BREAK | |||
| 8 | M | 3/21 | 3.2: Phenotyping Salk mutants |
| W | 3/23 | 5.1: Genotype Salk mutants (DNA extraction) Lab 4 report due |
|
| 9 | M | 3/28 | 5.2 Genotype Salk mutants (PCR) |
| W | 3/30 | 5.3 Genotype Salk mutants (gel purification) | |
| 10 | M | 4/04 | 5.4: Sequence analysis |
| W | 4/06 | 6.1: Gene expression resources Lab 5 report due |
|
| 11 | M | 4/11 | 6.2: Statistical analysis of gene expression data |
| W | 4/13 | 6.3: q-RT-PCR planning (see Planting schedule for prep) | |
| 12 | W | 4/20 | 6.4: RNA extraction and quantification |
| 13 | M | 4/25 | 6.5: RT and qPCR |
| W | 4/27 | catch-up day Lab 3 report due |
|
| 14 | M | 5/02 | 6.6: Analysis of qPCR data |
| W | 5/04 | Work on final presentation | |
| Finals | M | 5/09 | Lab 6 report due |
| W | 5/11 | Final presentation due Last chance for make-ups Extra credit due |
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