2.1 DNA extraction 2022

Submitted by kdorfman on Thu, 02/10/2022 - 14:21

Here are the equipment & reagents

LN2, tongs and dewars

ball mill (pre-chill the blocks)

grinding tubes and steel balls

miracloth cut into squares

funnels

Compost pot

Stinky waste bucket in hood for Beta mercapto ethanol

Dirty pots dishpan

Reagent per rxn per pair per class
DNA extraction buffer 600 uL 1250 uL 16.25 mL
KOAc 5M </a. 250 uL 550 uL 7.150 mL
Isopropanol 600 uL 1200 uL 15.6 mL - pre-aliquoted into round bottom centrifuge tubes
EtOH 70% 400 uL 850 uL 12 mL
NaOAc 3M 10 uL 25 uL 325 uL
T10E5 100 uL 225uL 3 mL
EtOH 100% 200 uL 450 uL 6 mL
T10E1 50 uL 125 uL 1.625 mL