!2022 (Maresca)

Submitted by kdorfman on Fri, 06/03/2022 - 18:13

Tom Maresca's first run of G&GA

Reagent for part # ordered? received?
1 M Tris HCl pH 7.5 Isothermal mix no on buffer shelf
1 M MgCl2 Isothermal mix no on buffer shelf
10 mM dNTP isothermal mix Fisher R9012 no in RT reagents box (-20)
1M DTT isothermal mix Acros 426380500 no in refrigerator door
Peg 8000 isothermal mix Fisher 04344322 yes no
NAD isothermal mix MP Biomedicals 02100499.1 yes
T5 exonuclease PCR Fisher M0663S yes Maresca freezer box
Phusion polymerase PCR Fisher F530S yes Maresca freezer box -20
Taq ligase PCR NEB M0208L yes Maresca freezer box
SiR Tubulin microtubule stain Fisher NC0958386 yes Maresca freezer box -20
T7 RiboMAX Express RNAi System RNAi expts Promega PRP1700 yes Maresca freezer box
Heat inactivated FBS S2 culture Fisher 10082147 yes freezer -20
Anti-anti S2 culture Fisher F530S no tissue culture aliquots freezer box
S2 medium S2 culture Fisher 21720-24 ordered 4 no
ZR plasmid miniprep S2 transformation D4015 yes