ConA dishes for 383H

Submitted by kdorfman on Wed, 09/21/2022 - 20:18

Materials:

  • con A aliquots

  • cleaned (but not necessarily sterile coverslip dishes (check size so they'll fit into the adaptors for the microscope stage)

  • 10 mL S2 medium in a conical tube

Protocol

  • Students put conA in non-sterile dishes

  • When they split their cultures, they put 1 mL of cells into a microfuge tube.

  • In class, they put cells onto the treated coverslip, then add medium after they are stuck.