Transcription mix
Students make the transcription mix from the kit
1 reaction per group
Reagent | Sample reaction |
---|---|
RiboMAX™ Express T7 2X Buffer | 10 µL |
linear DNA template (1µg total) | 1– 8µL |
Nuclease-Free Water | 0–7µl |
Enzyme Mix, T7 Express | 2 µL |
Final Volume | 20 µL |
- Printer-friendly version
- Log in to post comments