Immunofluorescence Labeling
Turn on the Incubator
Reagents
PBS (warm) for rinsing medium off cells before fixation
PBS-Tw-Az for rinsing coverslips (in carboy)
2% BSA in PBS to make antibodies
Fixative
- paraglut for students
- methanol in freezer for certain antibodies
Slides
Nail polish
Mystery Antibodies for 2012
No. |
Antibody |
from |
fixative |
secondary |
filter cube |
---|
1 |
Golgi |
KD |
formaldehyde |
goat anti mouse |
G |
2 |
LAP2 |
PW |
methanol |
goat anti mouse |
G |
3 |
gamma tub |
PW |
paraglut or form |
goat anti rabbit |
G |
4 |
ZO1 |
KD |
methanol or paraform |
goat anti rabbit |
G |
5 |
vinculin |
PW |
formaldehyde |
goat anti mouse |
G |