Extracting genomic DNA from Arabidopsis thaliana
Ingredient | per pair | per class |
---|---|---|
DEB | exactly 6 mL in round-bottomed tube | 100 mL |
add 5 µL BME per tube at the last minute | ||
T10E1 | 1 mL | 4 mL/pair/semester. Make 100 1mL aliquots |
T10E5 | 1 mL | 15 mL |
EtOH 95% | 1.5 mL | 50 mL |
EtOH 70% | 5 mL | 100 mL |
ISOprop | exactly 6 mL in round-bottom tube | 100 mL |
KOAc 5M | 4 mL | 50 mL |
NaOAc 3M pH 5.2 | 100 µL | 5 mL |
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