Iron experiment

Grow Col0 seeds to test effect of iron deprivation on gene expression

Make plates:

• 18 MS plates

• 6 iron-free MS plates

Sow sterile seeds on cellophane in 12 MS plates as heavily as possible.

After stratification,

• 7 days in incubator

• Transfer half to iron-free plates, half to MS plates

• Harvest after 3 days