Enzymes & kits

• Takara Ex-Taq
  giant economy size: RR001B 4x 250U $590 at Clontech
  $843 at Fisher

Fisher equivalent has this buffer: Tris-HCl 100mM, pH 9, KCl 500 mM, MgCl2 15 mM. Takara buffer is 20 mM MgCl2

• try this? cheap Taq at http://www.bulldog-bio.com/bioreadyrtaq.html

• Invitrogen 18080044 superscript III 200U/µL 10KU @ $259

• Invitrogen 10777019 RNAse out 5KU @ $143

• Invitrogen RNAse A Check supply.

• Qiagen 79254 DNAse
  includes RNase-free water and RDD for diluting the DNAse

• Qiagin RNEasy 74904

  • if needed RW1 1053394
  • if needed RLT 79216

• Quantifast 204054 400 rxns for q pcr

• Zymo Clean & Concentrator kit

• Oligo-dT Fisher FERSO131 Oligo dT 100 µM 60 µL @ ~$50. Dilute to 50µM!! (for emergencies: 50 µM oligo(dT) Fisher stockroom: C1101 0.5µg/µL x 40µL = 20µg)

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