Prepare a working solution of 1X Click-iT® EdU reaction buffer (Component D):
Transfer the solution (4 mL) in the Component D bottle to 36 mL of deionized water.
To make smaller amounts of 1X Click-iT® EdU reaction buffer,
dilute volumes from the Component D bottle 1:10 with deionized water.
After use, store any remaining 1X solution at 2–8˚C.
When stored as directed, this 1X solution is stable for 6 months.