SC -Leu High Ade 4X

Submitted by kdorfman on Sun, 01/26/2020 - 16:23

Using dilution factor 4:
(Because 10x won't go into solution)

But the agar is so viscous that bubbles don't reach the surface. Hard to pour.

To make
SC-leu high ade agar plates
with mm plate diameter
pour mL per plate
start with: mL water
add: g agar, leave stir bar in
autoclave for: minutes
Meanwhile, using X SC-AHLT
Asceptically mix together,
warm up, then add to autoclaved agar:		 mL SC-AHLT,
and : mL 10x YNB+AS ,
and: mL 20% glucose ,
and: mL 10X Adenine hemi sulfate,
and: mL 50X Tryptophan,
and: mL 100X Histidine HCl.
for a final volume of mL

Mark with one green line