Grind roots +/-, shoots +/- in ball mill (Sam's Retch Mixer Mill MM400)
Precool the white block for the ball mill
prepare 2 2-mL tubes per plate (label tubes on side as well as top)
- 3 roots + iron
- 3 roots - iron
- 3 shoots + iron
- 3 shoots - iron
2 metal beads per tube 3.2mm diameter ss beads (90g) - Cat. No. 11079132ss from www.biospec.com (Can also get from Fisher)
cool in LN2
Add tissue to cold tube, put back in LN2
Put all tubes in cold block
Shake 20 sec, check. The heat of shaking may start to defrost the tissue. Throw it back in the LN2. (Shake too long, and the cap may disintegrate!
Return to LN2
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