MS complete

Submitted by kdorfman on Mon, 12/19/2011 - 20:45
ingredient 750 800 1000 1200 1600 mL
number of plates 25 27 33 40 53
ddH2O* 450 480 600 720 960 mL
10X macronutrients 1 75 80 100 120 160 mL
10X micronutrients 2 75 80 100 120 160 mL
MES 0.375 0.4 0.5 0.6 0.8 g

*Add the other salt mixtures to the water to prevent precipitation

pH to 5.7 with 1M KOH (initial pH = ~3.66) (needs ~720 µL/L)

Bring to volume with distilled water

ingredient 750 800 1000 1200 1600 mL
bacto or phyto agar 7.5 8 10 12 16 g

autoclave with stir bar in flask or bottle

Stir until cool enough to handle

Pour 30 mL per plate (use the deep ones)

(Or Sigma M5524 - macro and micro nutrients together.)

  1. Sigma M 0654 Murashige and Skoog basal salt macronutrient solution (Krackeler 45-M0654-1L-EA) ~$26 ↩︎

  2. Sigma M 0529 Murashige and Skoog basal salt micronutrient solution (Krackeler 45-M0529-1L-EA) ~$26 ↩︎