**Murashige & Skoog 1µM iron medium
MS 10x micronutrients is 100 µM FeSO4, so MS complete is 10 µM
FeSO4 stock is 10 mM, which is 10,000x 1 µM
ingredient | 750 | 1000 | 1200 | 1500 | mL |
---|---|---|---|---|---|
number of plates | 25 | 33 | 40 | 50 | |
ddH2O* | 450 | 600 | 600 | 960 | mL |
10X macronutrients | 75 | 100 | 120 | 150 | mL |
boric acid 1000x | 0.75 | 1.0 | 1.2 | 1.5 | mL |
Cobalt chloride 10,000x | 0.075 | 0.1 | 0.12 | 0.15 | mL |
cupric sulfate 10,000x | 0.075 | 0.1 | 0.12 | 0.15 | mL |
ferrous sulfate 10,000x | 0.075 | 0.1 | 0.12 | 0.15 | mL |
KI 10,000x | 0.075 | 0.1 | 0.12 | 0.15 | mL |
manganese sulfate 1000x | 0.75 | 1.0 | 1.2 | 1.5 | mL |
molybdic acid 10,000x x | 0.075 | 0.1 | 0.12 | 0.15 | mL |
zinc sulfate 1000x | 0.75 | 1.0 | 1.2 | 1.5 | mL |
MES | 0.375 | 0.5 | 0.6 | 0.75 | g |
*Add the other salt mixtures to the water to prevent precipitation
pH to 5.7 with 1M KOH (initial pH = ~4.6) (needs ~570 drops/L)
Bring to volume with distilled water
ingredient | 750 | 1000 | 1200 | 1500 | mL |
---|---|---|---|---|---|
bacto or phyto agar | 7.5 | 10 | 12 | 15 | g |
autoclave with stir bar in flask or bottle
Stir until cool enough to handle
Pour 30 mL per plate (use the deep ones)
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