On plates

Planting on Plates

Make MS plates

• To get weakling mutants started before potting in soil
• Or to plant seeds on specified media
• Or to see roots

Seed Preparation

Seeds must be sterilized before they are put on sterile plates.

After sterilization,

• Make a line of seeds in agarose above the midline of the plate, either
  • either on the agar directly
  • on flattened cellophane
• Tape plates shut with Micropore surgical tape
• Stack plates flat
• Refrigerate the plates for 2 days in the dark
• Move plates to growth chamber
• Stand plates on edge under the lights. Tape several together.

0.1% agarose for plating seeds

• 100 mL sterile ddH2O
• 0.1 g agarose . autoclave to melt; shake bottle after autoclaving