hydroponics

http://www.plantmethods.com/content/9/1/4

http://www.bio-world.com/productinfo/3_44_292/124429/Magenta-GA-Plant-C…

2015 attempt

• Make 1x MS, pH to 5.6

• Split: most as growth medium, a little bit for 0.7% agar (err on the low end), sterilize

• Cut lids off microfuge tubes, poke a hole in each, sterilize in a beaker

• line lids up, flat side down, on a strip of scotch tape

• fill with 0.7% agar.

• put lids into floatie or other rack.

• put 1 sterilized seed in each hole. (in 0.1% agar)

• Wrap with saran wrap

• Cover tightly with foil, stratify in refrigerator for 2-3 days

• Remove foil, put container in growth chamber at 16h day, 22C

• Transfer lid to 50 mL tube with hole drilled in it at ~3 weeks.

• Aerate or stir medium